HIF1A Antibody - N-terminal region

Product Overview

• Article Name: HIF1A Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP30572_P050
• Supplier Catalog Number: ARP30572_P050
• Alternative Catalog Number: ASB-ARP30572_P050-25UL,ASB-ARP30572_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HIF1A
• Alternative Names: HIF1, MOP1, PASD8, HIF-1A, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF-1-alpha

Product Description

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). HIF1A is the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 93 kDa
• NCBI: 3091
• UniProt: Q16665
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-HIF1A antibody
Catalog Number: ARP30572
Paraffin Embedded Tissue: Human Heart cell Cellular Data: cardiac cell of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. The protein may be modified by phosphorylation.

WB Suggested Anti-HIF1A Antibody
Titration: 1.0 ug/ml
Positive Control: Fetal Heart

Rabbit Anti-HIF1A Antibody
Catalog Number: ARP30572_P050
Formalin Fixed Paraffin Embedded Tissue: Human Bone Marrow Tissue
Observed Staining: Nucleus, Cytoplasm
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

HIF1A antibody - N-terminal region (ARP30572_P050) in Human Fetal heart using Western Blot