Gabra5 Antibody - middle region

Product Overview

• Article Name: Gabra5 Antibody - middle region
• Biozol Catalog Number: ASB-ARP30687_P050
• Supplier Catalog Number: ARP30687_P050
• Alternative Catalog Number: ASB-ARP30687_P050-25UL,ASB-ARP30687_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide corresponding to the middle region of mouse GABRA5
• Alternative Names: A230018I05Rik

Product Description

GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 52 kDa
• NCBI: 110886
• UniProt: Q8BHJ7
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-Gabra5 Antibody
Titration: 1.0 ug/ml
Positive Control: Mouse liver

Host: Rabbit
Target Name: Gabra5
Sample Tissue: Mouse Liver
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: GABRA5
Sample Tissue: Mouse Liver
Antibody Dilution: 1ug/ml

25 ug of the indicated Mouse tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Full length 52 kDa Gabra5 is cleaved to a mature 48 kDa form, and the protein is also subject to N-linked glycosylation.

Host: Rabbit
Target Name: GABRA5
Sample Tissue: Mouse Brain
Antibody Dilution: 1ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

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