ZBTB32 Antibody - N-terminal region

Product Overview

• Article Name: ZBTB32 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP31874_P050
• Supplier Catalog Number: ARP31874_P050
• Alternative Catalog Number: ASB-ARP31874_P050-25UL,ASB-ARP31874_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ZBTB32
• Conjugation: Unconjugated
• Alternative Names: Rog, FAXF, FAZF, TZFP, ZNF538

Product Description

ZBTB32 may play an essential role during the proliferative stages of primitive hematopoietic progenitors, possibly acting in concert with (a subset of) the Fanconi anemia proteins. This gene can also interact with GATA-2 and can modify GATA-2 transactivation capacity.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 33 kDa
• NCBI: 27033
• UniProt: Q8WVP2
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-ZBTB32 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:2500
Positive Control: HepG2 cell lysate

Host: Rabbit
Target Name: WT1
Sample Type: 721_B
Antibody Dilution: 1.0ug/mlZBTB32 is supported by BioGPS gene expression data to be expressed in 721_B

Host: Rabbit
Target Name: ZBTB32
Sample Tissue: Human DLD1 Whole Cell
Antibody Dilution: 1ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

ZBTB32 antibody - N-terminal region (ARP31874_P050) in Human HepG2 using Western Blot