GLI2 Antibody - middle region

Product Overview

• Article Name: GLI2 Antibody - middle region
• Biozol Catalog Number: ASB-ARP31885_T100
• Supplier Catalog Number: ARP31885_T100
• Alternative Catalog Number: ASB-ARP31885_T100-25UL,ASB-ARP31885_T100-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GLI2
• Alternative Names: CJS, HPE9, PHS2, THP1, THP2

Product Description

GLI2 encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Molecular Weight: 133kDa
• NCBI: 2736
• UniProt: P10070
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-GLI2 Antibody
Catalog Number: ARP31885
Paraffin Embedded Tissue: Human Heart
Cellular Data: Myocardial cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

Rabbit Anti-GLI2 Antibody
Catalog Number: ARP31885
Paraffin Embedded Tissue: Human Intestine
Cellular Data: Epithelial cells of intestinal gland
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

Sample Type:
Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStopPrimary Antibody Dilution:
1:50Secondary Antibody:
Goat anti-rabbit-cy3Secondary Antibody Dilution:
1:1500Color/Signal Descriptions:
GLI2: Green Neural crest cells:: RedGene Name:
GLI2Submitted by:
Anonymous

Host: Rabbit
Target Name: GLI2
Sample Type: Jurkat
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 2.5ug/mL
Peptide Concentration: 4.0ug/mL
Lysate Quantity: 25ug/lane
Gel Concentration: 6%-18%

Host: Rabbit
Target Name: GLI2
Sample Type: HepG2 Whole Cell lysates
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: GLI2
Sample Tissue: Human RPMI 8226 Whole Cell
Antibody Dilution: 1ug/ml

GLI2 antibody - middle region (ARP31885_T100) in Jurkat using Western Blot