HDAC2 Antibody - middle region

Product Overview

• Article Name: HDAC2 Antibody - middle region
• Biozol Catalog Number: ASB-ARP31931_P050
• Supplier Catalog Number: ARP31931_P050
• Alternative Catalog Number: ASB-ARP31931_P050-25UL,ASB-ARP31931_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: ChIP, IHC, IP, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HDAC2
• Alternative Names: HD2, RPD3, YAF1, KDAC2

Product Description

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin struct

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 65kDa
• NCBI: 3066
• UniProt: Q92769-2
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Chromatin Immunoprecipitation (ChIP) Using HDAC2 antibody - middle region (ARP31931_P050) and HCT116 Cells

IHC Suggested Anti-HDAC2 antibody
Titration: 5ug/ ml
Positive Control: Breast

Rabbit Anti-HDAC2 Antibody
Catalog Number: ARP31931_P050
Formalin Fixed Paraffin Embedded Tissue: Human Testis Tissue
Observed Staining: Nucleus, Cytoplasm
Primary Antibody Concentration: 1:600
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

HDAC2 was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with ARP31931_P050 with 1:200 dilution. Western blot was performed using ARP31931_P050 at 1/1000 dilution.
Lane 1: Control IP in HEK293 Whole Cell Lysate.
Lane 2: HDAC2 IP with ARP31931_P050 in HEK293 Whole Cell Lysate.
Lane 3: Input of HEK293 Whole Cell Lysate.

WB Suggested Anti-HDAC2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: HT1080 cell lysate

HDAC2 antibody - middle region (ARP31931_P050) in Human HT1080 using Western Blot