SMAD4 Antibody - middle region

Product Overview

• Article Name: SMAD4 Antibody - middle region
• Biozol Catalog Number: ASB-ARP32005_T100
• Supplier Catalog Number: ARP32005_T100
• Alternative Catalog Number: ASB-ARP32005_T100-25UL,ASB-ARP32005_T100-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SMAD4
• Alternative Names: JIP, DPC4, MADH4, MYHRS

Product Description

SMAD4 is one of the Smad family members, which are essential intracellular signalling components of the transforming growth factor-beta (TGF-beta) superfamily. Smad2 and Smad3 are structurally highly similar and mediate TGF-beta signals. Smad4 is distantly related to Smads 2 and 3, and forms a heteromeric complex with Smad2 after TGF-beta or activin stimulation. TGF-beta induces heteromeric complexes of Smads 2, 3 and 4, and their concomitant translocation to the nucleus, which is required for efficient TGF-beta signal transduction

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Molecular Weight: 60 kDa
• NCBI: 4089
• UniProt: Q13485
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-SMAD4 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate

Sample Type: Human nuclear cell extracts (30ug)
Primary Dilution: 1:1000
Secondary Antibody: anti-Rabbit HRP
Secondary Dilution: 1:20000
Image Submitted By: AKatarina Luciakova
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SMAD4 antibody - middle region (ARP32005_T100) validated by WB using U2OS nuclear extracts

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 5 ug/mL of the antibody was used in this experiment. Peptide is present in 60 kDa, 53 kDa, and 35 kDa isoforms, and the protein may be sumoylated or ubiquitinated.

SMAD4 antibody - middle region (ARP32005_T100) in Human HepG2 using Western Blot