HEY1 Antibody - C-terminal region

Product Overview

• Article Name: HEY1 Antibody - C-terminal region
• Biozol Catalog Number: ASB-ARP32512_T100
• Supplier Catalog Number: ARP32512_T100
• Alternative Catalog Number: ASB-ARP32512_T100-25UL,ASB-ARP32512_T100-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HEY1
• Alternative Names: CHF2, OAF1, HERP2, HESR1, HRT-1, NERP2, hHRT1, BHLHb31

Product Description

Hairy/enhancer of split-related proteins, such as HEY1, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates.

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Molecular Weight: 33 kDa
• NCBI: 23462
• UniProt: Q9Y5J3
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-HEY1 Antibody
Catalog Number: ARP32512
Paraffin Embedded Tissue: Human Lung
Cellular Data: Alveolar cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

Rabbit Anti-HEY1 Antibody
Catalog Number: ARP32512
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

WB Suggested Anti-HEY1 Antibody
Titration: 2.5ug/ml
Positive Control: Lung

Host: Rabbit
Target Name: HEY1
Sample Tissue: Human 293T
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: HEY1
Sample Tissue: Human 293T Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: HEY1
Sample Tissue: Human ACHN Whole Cell
Antibody Dilution: 3ug/ml

Host: Rabbit
Target: HEY1
Positive control (+): NCI-H226 (N13)
Negative control (-): U937 (N31)
Antibody concentration: 1ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

HEY1 antibody - C-terminal region (ARP32512_T100) in Human 293T using Western Blot