HDAC2 Antibody - middle region

Product Overview

• Article Name: HDAC2 Antibody - middle region
• Biozol Catalog Number: ASB-ARP32619_P050
• Supplier Catalog Number: ARP32619_P050
• Alternative Catalog Number: ASB-ARP32619_P050-25UL,ASB-ARP32619_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: ChIP, IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HDAC2
• Alternative Names: HD2, RPD3, YAF1, KDAC2

Product Description

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly concerved from yeast to human.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 65kDa
• NCBI: 3066
• UniProt: Q92769
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Chromatin Immunoprecipitation (ChIP) Using HDAC2 antibody - middle region (ARP32619_P050) and HCT116 Cells

Rabbit Anti-HDAC2 antibody
Catalog Number: ARP32619_P050
Paraffin Embedded Tissue: Human Lung cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

WB Suggested Anti-HDAC2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 293T cell lysateHDAC2 is supported by BioGPS gene expression data to be expressed in HEK293T

Host: Rabbit
Target Name: HDAC2
Sample Type: Human Adult Placenta
Antibody Dilution: 1.0ug/ml

HDAC2 antibody - middle region (ARP32619_P050) in Human 293T using Western Blot