IKBKB Antibody - N-terminal region

Product Overview

• Article Name: IKBKB Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP32665_P050
• Supplier Catalog Number: ARP32665_P050
• Alternative Catalog Number: ASB-ARP32665_P050-25UL,ASB-ARP32665_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human IKBKB
• Alternative Names: IKK2, IKKB, IMD15, IMD15A, IMD15B, NFKBIKB, IKK-beta

Product Description

NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-203 AL708460.1 9-211 204-3077 AF080158.1 170-3043 3078-3916 AK023193.1 1980-2818

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 86kDa
• NCBI: 3551
• UniProt: O14920
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-IKBKB Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human brain

Researcher:Andreia Carvalho. Instituto de Biologia Molecular e Celular, Universidade do Porto (IBMC-UP)/Organelle Biogenesis and Function (OBF) GroupApplication:Western blottingSpecies+tissue/cell type:
Lane 1: 100ug mouse liver lysate
Lane 2: 100ug mouse brain lysate
Lane 3: 100ug mouse heart lysate
Lane 4: 100ug mouse kidney lysate
Lane 5: 100ug mouse lung lysate
Lane 6: 100ug mouse thymus lysate
Lane 7: 100ug mouse spleen lysate
Lane 8: 100ug mouse testis lysate
Lane 9: 100ug HeLa cell lysate
Primary antibody dilution:1:1000
Secondary antibody:Anti-rabbit-AP
Secondary antibody dilution:1:10,000

Host: Rabbit
Target Name: IKBKB
Sample Type: Human 721_B
Antibody Dilution: 1.0ug/mlIKBKB is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells

Sample Type: Primary vascular endothelial cellsProtocol: Loaded 30ug of cell lysate of each. One normal, one cultured with high glucose (which enhances IKK Beta). Expected band is cut. 10% Gel concentration.Primary dilution: 1:1000

IKBKB antibody - N-terminal region (ARP32665_P050) in Human brain using Western Blot