NKX6-1 Antibody - N-terminal region

Product Overview

• Article Name: NKX6-1 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP32720_P050
• Supplier Catalog Number: ARP32720_P050
• Alternative Catalog Number: ASB-ARP32720_P050-25UL,ASB-ARP32720_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NKX6-1
• Alternative Names: NKX6A, NKX6.1

Product Description

NKX6-1 is the transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. NKX6-1 is involved in transcriptional regulation in islet beta cells. Binds to the insulin promoter and is involved in regulation of the insulin gene. Together with NKX2-2 and IRX3, NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. NKX6-1 belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals.In the pancreas, NKX6.1 is required for the development of beta cells and is a potent bifunctional transcription regulator that binds to AT-rich sequences within the promoter region of target genes Iype et al. (2004) [PubMed 15056733].[supplied by OMIM]. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-300 U66797.1 1-300 301-307 AC096766.3 119394-119400 c 308-676 U66797.1 308-676 677-849 U66798.1 7-179 850-1116 U66799.1 7-273

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 38 kDa
• NCBI: 4825
• UniProt: P78426
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-NKX6-1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human Small Intestine

Host: Mouse
Target Name: NKX6-1
Sample Tissue: Mouse Liver
Antibody Dilution: 1ug/ml

Host: Rabbit
Target: NKX6-1
Positive control (+): 293T Cell Lysate (2T)
Negative control (-): HepG2 Cell Lysate (HG)
Antibody concentration: 1ug/ml

Host: Rabbit
Target Name: NKX6-1
Sample Tissue: Human MCF7 Whole Cell
Antibody Dilution: 1ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.

NKX6-1 antibody - N-terminal region (ARP32720_P050) in Human Small Intestine using Western Blot