OLIG2 Antibody - C-terminal region

Product Overview

• Article Name: OLIG2 Antibody - C-terminal region
• Biozol Catalog Number: ASB-ARP32753_P050
• Supplier Catalog Number: ARP32753_P050
• Alternative Catalog Number: ASB-ARP32753_P050-25UL,ASB-ARP32753_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Canine, Equine, Guinea pig, Human, Mouse, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human OLIG2
• Alternative Names: BHLHB1, OLIGO2, RACK17, PRKCBP2, bHLHe19

Product Description

OLIG2 is a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. OLIG2 is an essential regulator of ventral neuroectodermal progenitor cell fate. It is associated with T-cell acute lymphoblastic leukemia due to a chromosomal translocation t(14,21)(q11.2,q22). OLIG2 might play a role in learning deficits associated with Down syndrome.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 32 kDa
• NCBI: 10215
• UniProt: Q13516
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

OLIG2 Antibody - C-terminal region (ARP32753_P050) validated by WB using Fetal Lung cell lysate at 1.0ug/ml.

Host: Rabbit
Target Name: OLIG2
Sample Type: Fetal Lung
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1ug/ml
Peptide Concentration: 5ug/ml
Lysate Quantity: 25ug/lane
Gel Concentration: 0.12%

Host: Rabbit
Target Name: OLIG2
Sample Tissue: Human 293T
Antibody Dilution: 1.0ug/ml

Sample Type: Human Optic Nerve and Spinal CordCellular Target: Oligoden Drocyte Lineage Cells
Dilution: 1:500

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

OLIG2 Antibody - C-terminal region (ARP32753_P050) in Human 293T using Western Blot