ME1 Antibody - N-terminal region

Product Overview

• Article Name: ME1 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP32794_P050
• Supplier Catalog Number: ARP32794_P050
• Alternative Catalog Number: ASB-ARP32794_P050-25UL,ASB-ARP32794_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ME1
• Alternative Names: MES, HUMNDME

Product Description

ME1 is a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 64kDa
• NCBI: 4199
• UniProt: P48163
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. The peptide sequence is present in a 55 kDa isoform.

Rabbit Anti-ME1 Antibody
Catalog Number: ARP32794_P050
Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

WB Suggested Anti-ME1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate

Host: Rabbit
Target Name: ME1
Sample Type: 293T
Antibody Dilution: 1.0ug/mlME1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells

ME1 antibody - N-terminal region (ARP32794_P050) in Human HepG2 using Western Blot