PML Antibody - C-terminal region

Product Overview

• Article Name: PML Antibody - C-terminal region
• Biozol Catalog Number: ASB-ARP33048_P050
• Supplier Catalog Number: ARP33048_P050
• Alternative Catalog Number: ASB-ARP33048_P050-25UL,ASB-ARP33048_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PML
• Alternative Names: MYL, RNF71, PP8675, TRIM19

Product Description

PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. PML localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the proteins central and C-terminal regions, all variants encode the same N-terminus.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 59kDa
• NCBI: 5371
• UniProt: P29590
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Immunohistochemistry with Spleen cell lysate tissue at an antibody concentration of 5.0ug/ml using anti-PML antibody (ARP33048_P050)

Rabbit Anti-PML Antibody
Catalog Number: ARP33048_P050
Formalin Fixed Paraffin Embedded Tissue: Human Bone Marrow Tissue
Observed Staining: Nucleus, Cytoplasm
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

WB Suggested Anti-PML Antibody Titration: 1 ug/ml
Positive Control: Hela cell lysate

Host: Rabbit
Target Name: PML
Sample Type: Human Adult Placenta
Antibody Dilution: 1.0ug/ml

PML antibody - C-terminal region (ARP33048_P050) in Human HeLa using Western Blot