TBX20 Antibody - N-terminal region

Catalog Number: ASB-ARP33177_P050
Article Name: TBX20 Antibody - N-terminal region
Biozol Catalog Number: ASB-ARP33177_P050
Supplier Catalog Number: ARP33177_P050
Alternative Catalog Number: ASB-ARP33177_P050-25UL,ASB-ARP33177_P050-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: IHC, WB
Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TBX20
Alternative Names: ASD4
TBX20 is a member of the T-box transcription factor family expressed in the developing heart, eye, ventral neural tube, and limbs, indicating a possible role in regulating development of these tissues.
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 49kDa
NCBI: 57057
UniProt: Q9UMR3
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Human Muscle
Immunohistochemistry with Human kidney lysate tissue at an antibody concentration of 5.0ug/ml using anti-TBX20 antibody (ARP33177_P050)
25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 2 ug/mL of the antibody was used in this experiment.
Host: Rabbit
Target Name: TBX20
Sample Type: Jurkat cell lysates
Antibody Dilution: 1.0ug/ml
Host: Rabbit
Target Name: TBX20
Sample Tissue: Human HepG2
Antibody Dilution: 1.0ug/ml
Host: Rabbit
Target Name: TBX20
Sample Tissue: Human Hela Whole Cell
Antibody Dilution: 2ug/ml
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
TBX20 antibody - N-terminal region (ARP33177_P050) in Human HepG2 using Western Blot