PARP16 Antibody - middle region

Product Overview

• Article Name: PARP16 Antibody - middle region
• Biozol Catalog Number: ASB-ARP33752_P050
• Supplier Catalog Number: ARP33752_P050
• Alternative Catalog Number: ASB-ARP33752_P050-25UL,ASB-ARP33752_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PARP16
• Alternative Names: ARTD15, pART15, C15orf30

Product Description

Poly(ADP-ribosyl)ation is an immediate DNA-damage-dependent post-translational modification of histones and other nuclear proteins that contributes to the survival of injured proliferating cells. PARP16 is a member of poly(ADP-ribose) polymerases (PARPs) family that is encoded by different genes and displaying a conserved catalytic domain in which PARP-1 (113 kDa), the founding member, and PARP-2 (62 kDa) are so far the sole enzymes whose catalytic activity has been shown to be immediately stimulated by DNA strand breaks. A large repertoire of sequences encoding novel PARPs now extends considerably the field of poly(ADP-ribosyl)ation reactions to various aspects of the cell biology including cell proliferation and cell death. Some of these new members interact with each other, share common partners and common subcellular localizations suggesting possible fine tuning in the regulation of this post-translational modification of proteins.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 36 kDa
• NCBI: 54956
• UniProt: Q8N5Y8-3
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.

Rabbit Anti-PARP16 Antibody
Catalog Number: ARP33752_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy2/3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 a€'' 2.0 sec
Protocol located in Reviews and Data.

WB Suggested Anti-PARP16 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 293T cell lysatePARP16 is supported by BioGPS gene expression data to be expressed in HEK293T

Host: Rabbit
Target Name: PARP16
Sample Type: 293T
Antibody Dilution: 1.0ug/mlPARP16 is supported by BioGPS gene expression data to be expressed in HEK293T

Host: Rabbit
Target Name: PARP16
Sample Tissue: Human 293T
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target: PARP16
Positive control (+): Human Ovary (OV)
Negative control (-): Human stomach (ST)
Antibody concentration: 1ug/ml

PARP16 antibody - middle region (ARP33752_P050) in Human 293T using Western Blot