PARP2 Antibody - middle region

Product Overview

• Article Name: PARP2 Antibody - middle region
• Biozol Catalog Number: ASB-ARP33758_P050
• Supplier Catalog Number: ARP33758_P050
• Alternative Catalog Number: ASB-ARP33758_P050-25UL,ASB-ARP33758_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IF, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PARP2
• Alternative Names: ARTD2, ADPRT2, PARP-2, ADPRTL2, ADPRTL3, pADPRT-2

Product Description

This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 66 kDa
• NCBI: 10038
• UniProt: Q9UGN5
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Sample Type:
Rat thyrocytes-FRTL-5 Primary Antibody Dilution:
1:100 Secondary Antibody:
Anti-rabbit-Texas Red Secondary Antibody Dilution:
1:100 Color/Signal Descriptions:
Red: PARP2Blue: DAPI Gene Name:
PARP2Submitted by:
Syed A Morshed, Mount Sinai School of Medicine and James J Peters VA Medical Center

WB Suggested Anti-PARP2 Antibody Titration: 0.2-1 ug/ml
Positive Control: Raji cell lysatePARP2 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells

Host: Rabbit
Target Name: PARP2
Sample Tissue: Human Hela
Antibody Dilution: 1.0ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 0.5 ug/mL of the antibody was used in this experiment. The peptide is within isoform at 66 kDa, 65 kDa, and 58 kDa, also modified by phosphorylation.

Host: Rabbit
Target Name: PARP2
Sample Tissue: Human Jurkat Whole Cell
Antibody Dilution: 3ug/ml

Western Blot