NFKBIB Antibody - N-terminal region

Product Overview

• Article Name: NFKBIB Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP34382_P050
• Supplier Catalog Number: ARP34382_P050
• Alternative Catalog Number: ASB-ARP34382_P050-25UL,ASB-ARP34382_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NFKBIB
• Alternative Names: IKBB, TRIP9

Product Description

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664 or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).[supplied by OMIM].

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 38kDa
• NCBI: 4793
• UniProt: Q15653
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-NFKBIB Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:2500
Positive Control: Jurkat cell lysateNFKBIB is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells

Lanes:
Lane 1: 100ug mouse liver lysate
Lane 2: 100ug mouse brain lysate
Lane 3: 100ug mouse heart lysate
Lane 4: 100ug mouse kidney lysate
Lane 5: 100ug mouse lung lysate
Lane 6: 100ug mouse thymus lysate
Lane 7: 100ug mouse spleen lysate
Lane 8: 100ug mouse testis lysate
Lane 9: 100ug HeLa cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit-AP
Secondary Antibody Dilution:
1:10,000
Gene Name:
NFKBIB
Submitted by:
Andreia Carvalho. Instituto de Biologia Molecular e Celular, Universidade do Porto (IBMC-UP)/Organelle Biogenesis and Function (OBF) Group

Host: Rabbit
Target Name: NFKBIB
Sample Tissue: Mouse Liver
Antibody Dilution: 1ug/ml

Host: Mouse
Target Name: NFKBIB
Sample Tissue: Mouse Liver
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: NFKBIB
Sample Tissue: Human PANC1 Whole Cell
Antibody Dilution: 1ug/ml

NFKBIB antibody - N-terminal region (ARP34382_P050) in Human Jurkat using Western Blot