ZYX Antibody - middle region

Product Overview

• Article Name: ZYX Antibody - middle region
• Biozol Catalog Number: ASB-ARP34388_P050
• Supplier Catalog Number: ARP34388_P050
• Alternative Catalog Number: ASB-ARP34388_P050-25UL,ASB-ARP34388_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ZYX
• Alternative Names: ESP-2, HED-2

Product Description

TRIM50 is an E3 ubiquitin-protein ligase.Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 61kDa
• NCBI: 7791
• UniProt: Q15942
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-ZYX Antibody
Catalog Number: ARP34388_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult liver
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy2/3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 a€'' 2.0 sec
Protocol located in Reviews and Data.

Rabbit Anti-ZYX Antibody
Catalog Number: ARP34388_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
Observed Staining: Cytoplasmic (cytoskeleton)
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy2/3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 a€'' 2.0 sec
Protocol located in Reviews and Data.

Rabbit Anti-ZYX Antibody
Catalog Number: ARP34388_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic in mitochondria
Primary Antibody Concentration: N/A
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

WB Suggested Anti-ZYX Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Human Stomach

WB Suggested Anti-ZYX antibody Titration: 1 ug/mL
Sample Type: Human heart

ZYX antibody - middle region (ARP34388_P050) in Human Stomach using Western Blot