TRIM31 Antibody - N-terminal region

Product Overview

• Article Name: TRIM31 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP34717_P050
• Supplier Catalog Number: ARP34717_P050
• Alternative Catalog Number: ASB-ARP34717_P050-25UL,ASB-ARP34717_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Human, Mouse, Rabbit
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TRIM31
• Alternative Names: RNF, HCG1, HCGI, C6orf13

Product Description

TRIM31 encodes for a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to both the cytoplasm and the nucleus.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 48 kDa
• NCBI: 11074
• UniProt: Q9BZY9
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Human kidney

WB Suggested Anti-TRIM31 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Jurkat cell lysate

Host: Mouse
Target Name: TRIM31
Sample Tissue: Mouse Heart
Antibody Dilution: 1ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

TRIM31 antibody - N-terminal region (ARP34717_P050) in Human Jurkat using Western Blot