KCNN2 Antibody - C-terminal region

Product Overview

• Article Name: KCNN2 Antibody - C-terminal region
• Biozol Catalog Number: ASB-ARP35094_T100
• Supplier Catalog Number: ARP35094_T100
• Alternative Catalog Number: ASB-ARP35094_T100-25UL,ASB-ARP35094_T100-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Monkey, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human KCNN2
• Alternative Names: SK2, hSK2, SKCA2, KCa2.2, SKCa 2

Product Description

Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. The protein encoded by KCNN2 is activated before membrane hyperpolarization and is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. The encoded protein is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodulin-binding subunits. KCNN2 is a member of the KCNN family of potassium channel genes.

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Molecular Weight: 64kDa
• NCBI: 3781
• UniProt: Q9H2S1
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Sample Type:
Rhesus macaque spinal cordPrimary Antibody Dilution:
1:300Secondary Antibody:
Donkey anti Rabbit 488Secondary Antibody Dilution:
1:500Color/Signal Descriptions:
Green: KCNN2Gene Name:
KCNN2Submitted by:
Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School

Lanes:
Rat brain section
Primary Antibody Dilution:
1:500
Secondary Antibody:
Anti-rabbit-biotin, streptavidin-diaminobenzidine
Secondary Antibody Dilution:
1:500
Gene Name:
KCNN2
Submitted by:
Dr. Amiel Rosenkranz, Rosalind Franklin University

WB Suggested Anti-KCNN2 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate

Host: Rabbit
Target Name: KCNN2
Sample Type: HepG2
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 2.5ug/mL
Peptide Concentration: 2.0ug/mL
Lysate Quantity: 25ug/lane
Gel Concentration: 12%

Host: Rabbit
Target Name: KCNN2
Sample Tissue: Human Fetal Liver
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target: KCNN2
Positive control (+): Human liver (LI)
Negative control (-): 293T (2T)
Antibody concentration: 0.5ug/ml

KCNN2 antibody - C-terminal region (ARP35094_T100) in Human Fetal Liver using Western Blot