SNRNP200 Antibody - N-terminal region

Product Overview

• Article Name: SNRNP200 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP36414_P050
• Supplier Catalog Number: ARP36414_P050
• Alternative Catalog Number: ASB-ARP36414_P050-25UL,ASB-ARP36414_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
• Alternative Names: BRR2, RP33, HELIC2, ASCC3L1, U5-200KD

Product Description

Pre-mRNA splicing is catalyzed by the spliceosome, a complex of specialized RNA and protein subunits that removes introns from a transcribed pre-mRNA segment. The spliceosome consists of small nuclear RNA proteins (snRNPs) U1, U2, U4, U5 and U6, together with approximately 80 conserved proteins. U5 snRNP contains nine specific proteins. This gene encodes one of the U5 snRNP-specific proteins. This protein belongs to the DEXH-box family of putative RNA helicases. It is a core component of U4/U6-U5 snRNPs and appears to catalyze an ATP-dependent unwinding of U4/U6 RNA duplices. Mutations in this gene cause autosomal-dominant retinitis pigmentosa.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 244kDa
• NCBI: 23020
• UniProt: O75643
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-SNRNP200 Antibody
Titration: 1.0 ug/ml
Positive Control: COLO205 Whole Cell

Host: Rabbit
Target Name: SNRNP200
Sample Tissue: Human A549 Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: SNRNP200
Sample Tissue: Human HCT116 Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: SNRNP200
Sample Tissue: Human Jurkat Whole Cell
Antibody Dilution: 1ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

SNRNP200 antibody - N-terminal region (ARP36414_P050) in Human COLO205 using Western Blot