TWNK Antibody - middle region

Product Overview

• Article Name: TWNK Antibody - middle region
• Biozol Catalog Number: ASB-ARP36483_P050
• Supplier Catalog Number: ARP36483_P050
• Alternative Catalog Number: ASB-ARP36483_P050-25UL,ASB-ARP36483_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PEO1
• Alternative Names: PEO, PEO1, SCA8, ATXN8, IOSCA, PEOA3, SANDO, TWINL, MTDPS7, PRLTS5, C10orf2

Product Description

This gene encodes a hexameric DNA helicase which unwinds short stretches of double-stranded DNA in the 5 to 3 direction and, along with mitochondrial single-stranded DNA binding protein and mtDNA polymerase gamma, is thought to play a key role in mtDNA replication. The protein localizes to the mitochondrial matrix and mitochondrial nucleoids. Mutations in this gene cause infantile onset spinocerebellar ataxia (IOSCA) and progressive external ophthalmoplegia (PEO) and are also associated with several mitochondrial depletion syndromes. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 77 kDa
• NCBI: 56652
• UniProt: Q96RR1
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. An isoform containing the peptide sequence is present at ~60 kDa.

WB Suggested Anti-PEO1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysate

Host: Rabbit
Target Name: PEO1
Sample Type: Hela
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1ug/ml
Peptide Concentration: 5.0 ug/ml
Lysate Quantity: 25ug/lane/lane
Gel Concentration: 12%C10orf2 is supported by BioGPS gene expression data to be expressed in Hela

Host: Rabbit
Target: C10ORF2
Positive control (+): HepG2 (HG)
Negative control (-): Human stomach (ST)
Antibody concentration: 1ug/ml

Host: Rabbit
Target Name: C10orf2
Sample Tissue: Human 786-0 Whole Cell
Antibody Dilution: 1ug/ml

PEO1 antibody - middle region (ARP36483_P050) in Human Jurkat using Western Blot