ANXA1 Antibody - N-terminal region

Product Overview

• Article Name: ANXA1 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP36568_T100
• Supplier Catalog Number: ARP36568_T100
• Alternative Catalog Number: ASB-ARP36568_T100-25UL,ASB-ARP36568_T100-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Guinea pig, Human, Mouse, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ANXA1
• Alternative Names: ANX1, LPC1

Product Description

ANXA1 encodes a protein that belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity.

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Molecular Weight: 39 kDa
• NCBI: 301
• UniProt: P04083
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

Human Intestine

WB Suggested Anti-ANXA1 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate

WB Suggested Anti-ANXA1 Antibody
Positive Control: Lane 1: 20ug mouse fibroblast lysates
Primary Antibody Dilution : 1:600
Secondary Antibody : Anti rabbit-HRP
Secondry Antibody Dilution : 1:2000
Submitted by: Anonymous

Host: Rabbit
Target Name: ANXA1
Sample Type: HepG2
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 2.5ug/mL
Peptide Concentration: 2.0ug/mL
Lysate Quantity: 25ug/lane
Gel Concentration: 12%

Host: Rabbit
Target Name: ANXA1
Sample Tissue: Human ACHN
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target: ANXA1
Positive control (+): A549 (N03)
Negative control (-): RPMI-8226 (N12)
Antibody concentration: 0.5ug/ml

ANXA1 antibody - N-terminal region (ARP36568_T100) in mouse fibroblasts using Western Blot