Atp6v0a1 Antibody - C-terminal region

Product Overview

• Article Name: Atp6v0a1 Antibody - C-terminal region
• Biozol Catalog Number: ASB-ARP37232_P050
• Supplier Catalog Number: ARP37232_P050
• Alternative Catalog Number: ASB-ARP37232_P050-25UL,ASB-ARP37232_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Equine, Human, Mouse, Porcine, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse
• Alternative Names: V, Vp, Atp, Vpp1, Vpp-1, ATP6a1, Atp6n1, Atp6n1a, Atpv0a1, AA959968, ARP37232

Product Description

Atp6v0a1 is required for assembly and activity of the vacuolar ATPase. Atp6v0a1 has a potential role in differential targeting and regulation of the enzyme for a specific organelle.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 96 kDa
• NCBI: 11975
• UniProt: A2A5A0
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Sample Type:
A. untransfected HeLa cells B. mATP6V0A1 transfected HeLa cellsPrimary Antibody Dilution:
1:250Secondary Antibody:
Anti-rabbit AlexaFluor 488Secondary Antibody Dilution:
1:1000Color/Signal Descriptions:
Atp6v0a1: Green DAPI:BlueGene Name:
Atp6v0a1 Submitted by:
Anonymous

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 0.5 ug/mL of the antibody was used in this experiment.

Host: Rabbit
Target Name: Atp6v0a1
Sample Tissue: Human DLD1 Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: Atp6v0a1
Sample Tissue: Mouse Mouse Skeletal Muscle
Antibody Dilution: 1ug/ml

WB Suggested Anti-Atp6v0a1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Mouse Uterus

Application: Western blotting
Species+tissue/cell type: HeLa cells
How many ugsof tissue/cell lysate run on the gel:1. 10 ug untransfected HeLa lysate2. 10 ug mATP6V0A2 (Partial) transfected HeLa lysate3. 10 ug mATP6V0A2-FLAG transfected HeLa lysate4. 10 ug mATP6V0A1-FLAG transfected HeLa lysate
Primary antibody dilution: 1:300
Secondary antibody: Anti-rabbit-HRP
Secondary antibody dilution: 1:1000

Atp6v0a1 antibody - C-terminal region (ARP37232_P050) in Human DLD1 Whole Cell using Western Blot