WT1 Antibody - middle region

Product Overview

• Article Name: WT1 Antibody - middle region
• Biozol Catalog Number: ASB-ARP37989_P050
• Supplier Catalog Number: ARP37989_P050
• Alternative Catalog Number: ASB-ARP37989_P050-25UL,ASB-ARP37989_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human WT1
• Alternative Names: GUD, AWT1, WAGR, WT33, NPHS4, WIT-2

Product Description

WT1 is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a sm

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 49 kDa
• NCBI: 7490
• UniProt: P19544
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Human Testis

WB Suggested Anti-WT1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: HT1080 cell lysate

Host: Rabbit
Target Name: WT1
Sample Tissue: Human Jurkat
Antibody Dilution: 1.0ug/ml

Host: Mouse
Target Name: WT1
Sample Tissue: Mouse Brain
Antibody Dilution: 1ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. The middle peptide is contained within multiple isoforms of this protein from 63 kDa to 33 kDa (~10 isoforms), and at least 4 of these isoforms seem represented in samples shown (63 kDa, 47-49 kDa, 40 kDa, and 33 kDa).

WT1 antibody - middle region (ARP37989_P050) in Mouse Brain using Western Blot