MXD1 Antibody - N-terminal region

Product Overview

• Article Name: MXD1 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP38141_P050
• Supplier Catalog Number: ARP38141_P050
• Alternative Catalog Number: ASB-ARP38141_P050-25UL,ASB-ARP38141_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MXD1
• Alternative Names: MAD, MAD1, BHLHC58

Product Description

MAX dimerization protein belongs to a subfamily of MAX-interacting proteins. MXD1 competes with MYC for binding to MAX to form a sequence-specific DNA-binding complex, acts as a transcriptional repressor (while MYC appears to function as an activator) and is a candidate tumor suppressor.MAX dimerization protein belongs to a subfamily of MAX-interacting proteins. This protein competes with MYC for binding to MAX to form a sequence-specific DNA-binding complex, acts as a transcriptional repressor (while MYC appears to function as an activator) and is a candidate tumor suppressor. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 25 kDa
• NCBI: 4084
• UniProt: Q0VFI9
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-MXD1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Human Muscle

Host: Mouse
Target Name: MXD1
Sample Tissue: Mouse Small Intestine
Antibody Dilution: 1ug/ml

Host: Rabbit
Target: MXD1
Positive control (+): Mouse small intestine (M-IN)
Negative control (-): Mouse testis (M-TE)
Antibody concentration: 1ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Reccommended dilution for this antibody is 1-3 ug/mL.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

MXD1 antibody - N-terminal region (ARP38141_P050) in Human Muscle using Western Blot