PPARGC1A Antibody - N-terminal region

Product Overview

• Article Name: PPARGC1A Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP39015_P050
• Supplier Catalog Number: ARP39015_P050
• Alternative Catalog Number: ASB-ARP39015_P050-25UL,ASB-ARP39015_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PPARGC1A
• Alternative Names: LEM6, PGC1, PGC1A, PGC-1v, PPARGC1, PGC-1alpha, PGC-1(alpha)

Product Description

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 91 kDa
• NCBI: 10891
• UniProt: Q9UBK2
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-PPARGC1A Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: ACHN cell lysate

PPARGC1A antibody - N-terminal region (ARP39015_P050) validated by WB using Transfected Melanoma Cell at 1:1000.

PPARGC1A antibody - N-terminal region (ARP39015_P050) validated by WB using transfected SH-SY5Y lysate at 1:15000.

Lanes:
Lane1: 30 ug Human liver
Lane2: 30 ug Rat liver
Lane3: 30 ug Mouse (wild-type) liver
Lane4: 30 ug Mouse [AMPK alpha 1/2(-/-)] liver
Lane5: 30 ug Human muscle
Lane6: 30 ug Rat muscle
Lane7: 30 ug Mouse muscle
Primary Antibody Dilution:
1:1000
Secondary Antibody:

Secondary Antibody Dilution:
1:10000
Gene Name:
PPARGC1A
Submitted by:
Dr. Bruno Guigas, PhD, Dept. of Molecular Cell Biology, Leiden University Medical Center

25 ug of the indicated Mouse whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Recommended dilution for antibody is 1-3 ug/mL.

Western Blot