BRD4 Antibody - C-terminal region

Product Overview

• Article Name: BRD4 Antibody - C-terminal region
• Biozol Catalog Number: ASB-ARP39076_P050
• Supplier Catalog Number: ARP39076_P050
• Alternative Catalog Number: ASB-ARP39076_P050-25UL,ASB-ARP39076_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: ChIP, IF, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human BRD4
• Alternative Names: CAP, MCAP, HUNK1, HUNKI

Product Description

BRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting.The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15,19)(q13,p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 152 kDa
• NCBI: 23476
• UniProt: O60885-2
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Chromatin Immunoprecipitation (ChIP) Using BRD4 antibody - C-terminal region (ARP39076_P050) and HCT116 Cells

Sample Type:
HeLa
Primary Antibody Dilution:
4 ug/ml
Secondary Antibody:
Anti-rabbit Alexa 546
Secondary Antibody Dilution:
2 ug/ml
Gene Name:
BRD4

WB Suggested Anti-BRD4 Antibody Titration: 0.2-1 ug/ml
Positive Control: Human Thymus

Host: Rabbit
Target Name: BRD4
Sample Tissue: Human 293T Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: BRD4
Sample Tissue: Human A549 Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: BRD4
Sample Tissue: Human Jurkat Whole Cell
Antibody Dilution: 1ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Peptide sequence is contained within Isoform A (152 kDa), Isoform B (88 kDa) and isoform C (80 kDa).

BRD4 antibody - C-terminal region (ARP39076_P050) in Human Thymus using Western Blot