GAPDH Antibody - N-terminal region
Catalog Number:
ASB-ARP40175_P050
- Images (9)
| Article Name: | GAPDH Antibody - N-terminal region |
| Biozol Catalog Number: | ASB-ARP40175_P050 |
| Supplier Catalog Number: | ARP40175_P050 |
| Alternative Catalog Number: | ASB-ARP40175_P050-25UL,ASB-ARP40175_P050-100UL |
| Manufacturer: | Aviva |
| Host: | Rabbit |
| Category: | Proteine/Peptide |
| Application: | IHC, WB |
| Species Reactivity: | Bovine, Canine, Equine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Sheep |
| Immunogen: | The immunogen is a synthetic peptide directed towards the N terminal region of human GAPDH |
| Alternative Names: | G3PD, GAPD, HEL-S-162eP |
| GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
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25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. An isoform containing the peptide sequence is present at 84 kDa. |
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Rabbit Anti-GAPDH Antibody Catalog Number: ARP40175_P050 Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue Observed Staining: Cytoplasm in hepatocytes Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
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Rabbit Anti-GAPDH Antibody Catalog Number: ARP40175_P050 Formalin Fixed Paraffin Embedded Tissue: Human Heart Muscle Tissue Observed Staining: Cytoplasm Primary Antibody Concentration: 1:600 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
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Host: Rabbit Target Name: GAPDH Sample Type: Hela Whole Cell lysates Antibody Dilution: 1.0ug/mlGAPDH is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells |
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WB Suggested Anti-GAPDH antibody Titration: 1 ug/mL Sample Type: Human Raji |
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WB Suggested Anti-GAPDH Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Human brain |
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Lanes: 1. 50 ug NIH3T3 cytosolic extract 2. 50 ug NIH3T3 cytosolic extract 3. 50 ug NIH3T3 cytosolic extract 4. 50 ug NIH3T3 cytosolic extract Primary Antibody Dilution: 1:1000 Secondary Antibody: Anti-Rabbit HRP Secondary Antibody Dilution: 1:30000 Gene Name: GAPDH Submitted by: Dr. Arimdam Basu, Dept. of Animal Biology, University of Pennsylvania |
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Host: Rabbit Target Name: GAPDH Sample Type: 293T Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1ug/ml Peptide Concentration: 5ug/ml Lysate Quantity: 25ug/lane/lane Gel Concentration: 0.12 |
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GAPDH antibody - N-terminal region (ARP40175_P050) in Human HeLa using Western Blot |
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