GAPDH Antibody - middle region

Product Overview

• Article Name: GAPDH Antibody - middle region
• Biozol Catalog Number: ASB-ARP40176_P050
• Supplier Catalog Number: ARP40176_P050
• Alternative Catalog Number: ASB-ARP40176_P050-25UL,ASB-ARP40176_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GAPDH
• Alternative Names: G3PD, GAPD, HEL-S-162eP

Product Description

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 36kDa
• NCBI: 2597
• UniProt: P04406
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-GAPDH Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Transfected 293T

Lanes:
Lane 1-7: 30 ug rat heart extract
Primary Antibody Dilution:
1:2000
Secondary Antibody:
Anti-Rabbit HRP
Secondary Antibody Dilution:
1:3000
Gene Name:
GAPDH
Submitted by:
Yanfei QI, University of Florida

WB Suggested Anti-GAPDH antibody Titration: 1 ug/mL
Sample Type: Human Raji

Host: Rabbit
Target Name: GAPDH
Sample Tissue: Rat Brain
Antibody Dilution: 1ug/ml

Host: Rat
Target Name: GAPDH
Sample Tissue: Rat Brain
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: GAPDH
Sample Tissue: Mouse Heart
Antibody Dilution: 1ug/ml

Host: Mouse
Target Name: GAPDH
Sample Tissue: Mouse Heart
Antibody Dilution: 1ug/ml

GAPDH antibody - middle region (ARP40176_P050) in Transfected 293T using Western Blot