SF3B1 Antibody - N-terminal region

Product Overview

• Article Name: SF3B1 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP40249_T100
• Supplier Catalog Number: ARP40249_T100
• Alternative Catalog Number: ASB-ARP40249_T100-25UL,ASB-ARP40249_T100-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SF3B1
• Alternative Names: MDS, PRP10, Hsh155, PRPF10, SAP155, SF3b155

Product Description

SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the introns branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures.This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the introns branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Molecular Weight: 146 kDa
• NCBI: 23451
• UniProt: O75533
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-SF3B1 Antibody
Catalog Number: ARP40249
Paraffin Embedded Tissue: Human Liver
Cellular Data: Hepatocytes
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

Rabbit Anti-SF3B1 Antibody
Catalog Number: ARP40249
Paraffin Embedded Tissue: Human Skin
Cellular Data: Epidermal cells
Antibody Concentration: 16 ug/ml
Magnification: 400X

Rabbit Anti-SF3B1 Antibody
Catalog Number: ARP40249
Paraffin Embedded Tissue: Human alveolar cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

WB Suggested Anti-SF3B1 Antibody Titration: 1.25ug/ml
Positive Control: Human Thymus

Host: Rabbit
Target Name: SF3B1
Sample Tissue: Human MCF7
Antibody Dilution: 1.0ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. The peptide sequence is also in an isoform of 54 kDa and the protein may be modified via citrullination, sumoylation, and/or phosphorylation.

SF3B1 antibody - N-terminal region (ARP40249_T100) in Human MCF7 using Western Blot