EIF4G2 Antibody - C-terminal region

Product Overview

• Article Name: EIF4G2 Antibody - C-terminal region
• Biozol Catalog Number: ASB-ARP40361_P050
• Supplier Catalog Number: ARP40361_P050
• Alternative Catalog Number: ASB-ARP40361_P050-25UL,ASB-ARP40361_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human EIF4G2
• Alternative Names: P97, AAG1, DAP5, NAT1

Product Description

Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. EIF4G2 shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3, eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, EIF4G2 functions as a general repressor of translation by forming translationally inactive complexes.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 39kDa
• NCBI: 1982
• UniProt: P78344
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-EIF4G2 Antibody
Catalog Number: ARP40361_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: N/A
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

WB Suggested Anti-EIF4G2 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate

Sample Type: 1. IAH30 cells ,(0.5x106 ,cells loaded)
2. DF-1 cells (0.5x106 cells loaded)
Primary Dilution: 1:1000 ,
Secondary Antibody: Goat anti-rabbit IgG (whole molecule) peroxidase ,
Secondary Dilution:1:10,000 ,
Image Submitted By: Yongxiu Yao
Institute for Animal Health ,

EIF4G2 antibody - C-terminal region (ARP40361_P050) in Human HepG2 using Western Blot