ACO1 Antibody - N-terminal region

Product Overview

• Article Name: ACO1 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP40390_P050
• Supplier Catalog Number: ARP40390_P050
• Alternative Catalog Number: ASB-ARP40390_P050-25UL,ASB-ARP40390_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACO1
• Alternative Names: IRP1, ACONS, HEL60, IREB1, IREBP, IREBP1

Product Description

ACO1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). It plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5 UTR of ferritin mRNA, and in the 3 UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 98kDa
• NCBI: 48
• UniProt: P28271
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Host: Rabbit
Target Name: ACO1
Sample Tissue: Human Ovary Tumor
Antibody Dilution: 1ug/ml

Rabbit Anti-ACO1 Antibody
Catalog Number: ARP40390
Paraffin Embedded Tissue: Human Muscle
Cellular Data: Skeletal muscle cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

Sample Type:
Human Capan1 cells (Pancreatic cancer cell line)Primary Antibody Dilution:
1:300Secondary Antibody:
Anti-rabbit-AlexaFluor-488Secondary Antibody Dilution:
1:200Color/Signal Descriptions:
ACO1: Green DAPI: BlueGene Name:
ACO1Submitted by:
Dr. Pankaj Singh, UNMC, Omaha, NE

WB Suggested Anti-ACO1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human kidney

Lanes:
1. 60 ug mouse liver extract 2. 60 ug mouse liver extract 3. 60 ug mouse liver extract
Primary Antibody Dilution:
1:500
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:3000
Gene Name:
ACO1
Submitted by:
Dr. Hao Zhu, University of Kansas Medical Center

Lanes:
1. 45ug capan1 cell lysate
2. 45 ug HPAF cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-Rabbit HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
ACO1
Submitted by:
Dr. Pankaj Singh, UNMC, Omaha, NE

Lane 1: 100ug HePG2 lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Goat anti rabbit-HRP
Secondary Antibody Dilution:
1:8000
Gene Name:
ACO1
Submitted by:
JosAfA Antonio BAfArcena, University of CAfArdoba

Host: Rabbit
Target Name: ACO1
Sample Type: Fetal Kidney
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1ug/ml
Peptide Concentration: 5ug/ml
Lysate Quantity: 25ug/lane/Lane
Gel Concentration: 0.12

ACO1 antibody - N-terminal region (ARP40390_P050) in Human Ovary Tumor using Western Blot