XPOT Antibody - middle region

Product Overview

• Article Name: XPOT Antibody - middle region
• Biozol Catalog Number: ASB-ARP40711_P050
• Supplier Catalog Number: ARP40711_P050
• Alternative Catalog Number: ASB-ARP40711_P050-25UL,ASB-ARP40711_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, IP, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human XPOT
• Alternative Names: XPO3

Product Description

XPOT belonging to the RAN-GTPase exportin family that mediates export of tRNA from the nucleus to the cytoplasm. Translocation of tRNA to the cytoplasm occurs once exportin has bound both tRNA and GTP-bound RAN.This gene encodes a protein belonging to the RAN-GTPase exportin family that mediates export of tRNA from the nucleus to the cytoplasm. Translocation of tRNA to the cytoplasm occurs once exportin has bound both tRNA and GTP-bound RAN.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 110kDa
• NCBI: 11260
• UniProt: O43592
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. The peptide sequence is present in a 57 kDa isoform.

Rabbit Anti-XPOT Antibody
Catalog Number: ARP40711_P050
Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
Observed Staining: Cytoplasmic and nuclear
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1/600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

XPOT was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with ARP40711_P050 with 1:200 dilution. Western blot was performed using ARP40711_P050 at 1/1000 dilution.
Lane 1: Control IP in HEK293 Whole Cell Lysate.
Lane 2: XPOT IP with ARP40711_P050 in HEK293 Whole Cell Lysate.
Lane 3: Input of HEK293 Whole Cell Lysate.

WB Suggested Anti-XPOT Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysateXPOT is supported by BioGPS gene expression data to be expressed in Jurkat

XPOT antibody - middle region (ARP40711_P050) in Human Jurkat using Western Blot