NONO Antibody - C-terminal region

Product Overview

• Article Name: NONO Antibody - C-terminal region
• Biozol Catalog Number: ASB-ARP40716_T100
• Supplier Catalog Number: ARP40716_T100
• Alternative Catalog Number: ASB-ARP40716_T100-25UL,ASB-ARP40716_T100-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IF, IHC, IP, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NONO
• Alternative Names: P54, NMT55, NRB54, MRXS34, P54NRB, PPP1R114

Product Description

NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Molecular Weight: 52kDa
• NCBI: 4841
• UniProt: Q15233
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Sample Type : subnuclear bodies-paraspeckles

Human Liver

Human kidney

Rabbit Anti-NONO Antibody
Catalog Number: ARP40716
Paraffin Embedded Tissue: Human cardiac cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

NONO was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with ARP40716_T100 with 1:200 dilution. Western blot was performed using ARP40716_T100 at 1/1000 dilution.
Lane 1: Control IP in HEK293 Whole Cell Lysate.
Lane 2: NONO IP with ARP40716_T100 in HEK293 Whole Cell Lysate.
Lane 3: Input of HEK293 Whole Cell Lysate.

WB Suggested Anti-NONO Antibody Titration: 1.25ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate

Rabbit Anti-NONO Antibody
Catalog Number: ARP40716
Paraffin Embedded Tissue: Human cardiac cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

NONO antibody - C-terminal region (ARP40716_T100) in Human HepG2 using Western Blot