DMBT1 Antibody - N-terminal region

Product Overview

• Article Name: DMBT1 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP42352_P050
• Supplier Catalog Number: ARP42352_P050
• Alternative Catalog Number: ASB-ARP42352_P050-25UL,ASB-ARP42352_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DMBT1
• Alternative Names: SAG, GP340, SALSA, muclin

Product Description

Loss of sequences from human chromosome 10q has been associated with the progression of human cancers. The gene DMBT1 was originally isolated based on its deletion in a medulloblastoma cell line. DMBT1 is expressed with transcripts of 6.0, 7.5, and 8.0 kb in fetal lung and with one transcript of 8.0 kb in adult lung, although the 7.5 kb transcript has not been characterized. The DMBT1 protein is a glycoprotein containing multiple scavenger receptor cysteine-rich (SRCR) domains separated by SRCR-interspersed domains (SID). Transcript variant 2 (8.0 kb) has been shown to bind surfactant protein D independently of carbohydrate recognition. This indicates that DMBT1 may not be a classical tumor supressor gene, but rather play a role in the interaction of tumor cells and the immune system.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 258kDa
• NCBI: 1755
• UniProt: Q9UGM3
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-DMBT1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Human Stomach

Lanes:
Lane 1: 2ug MCF7-DMBT1+Dox
Lane 2: 2ug MCF7-DMBT1 -Dox
Lane 3: 2ug MCF7-Ctrl+Dox
Lane 4: 2ug MCF7-DMBT1 -Dox
Primary Antibody Dilution:
1:5000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:10,000
Gene Name:
DMBT1
Submitted by:
Matthias Rauen, Lundbeckfonden Center of Excellence NanoCAN, University of Southern Denmark

Host: Rabbit
Target: DMBT1
Positive control (+): Human Lung (LU)
Negative control (-): HeLa Cell Lysate (HL)
Antibody concentration: 1ug/ml

Host: Rabbit
Target Name: DMBT1
Sample Tissue: Human Human Stomach
Antibody Dilution: 1ug/ml

Host: Rabbit
Target Name: DMBT1
Sample Tissue: Human HepG2 Whole Cell
Antibody Dilution: 3ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

DMBT1 antibody - N-terminal region (ARP42352_P050) in Human Stomach using Western Blot