ATP6V0A2 Antibody - N-terminal region

Product Overview

• Article Name: ATP6V0A2 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP42365_P050
• Supplier Catalog Number: ARP42365_P050
• Alternative Catalog Number: ASB-ARP42365_P050-25UL,ASB-ARP42365_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rat, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ATP6V0A2
• Alternative Names: A2, RTF, TJ6, WSS, a2V, ARCL, J6B7, STV1, TJ6M, TJ6S, VPH1, ARCL2A, ATP6A2, ATP6N1D

Product Description

The multisubunit vacuolar-type proton pump (H(+)-ATPase or V-ATPase) is essential for acidification of diverse cellular components, including endosomes, lysosomes, clathrin-coated vesicles, secretory vesicles, and chromaffin granules, and it is found at high density in the plasma membrane of certain specialized cells. H(+)-ATPases are comprised of a peripheral V(1) domain and an integral membrane V(0) domain, ATP6V0A2 is a component of the V(0) domain.The multisubunit vacuolar-type proton pump (H(+)-ATPase or V-ATPase) is essential for acidification of diverse cellular components, including endosomes, lysosomes, clathrin-coated vesicles, secretory vesicles, and chromaffin granules, and it is found at high density in the plasma membrane of certain specialized cells. H(+)-ATPases are comprised of a peripheral V(1) domain and an integral membrane V(0) domain, ATP6V0A2 is a component of the V(0) domain (Smith et al., 2003 [PubMed 14580332]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 98kDa
• NCBI: 23545
• UniProt: Q9Y487
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Immunohistochemistry with Human kidney lysate tissue at an antibody concentration of 5.0ug/ml using anti-ATP6V0A2 antibody (ARP42365_P050)

Anti-ATP6V0A2 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Anti-ATP6V0A2 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Application: IHC/Immunofluorescence
Species+tissue/cell type:A. untransfected HeLa cellsB. mATP6V0A2-FLAG transfected HeLa cellsC. mATP6V0A2 (partial) transfected HeLa cells
Primary antibody dilution: 1:250
Secondary antibody: Anti-rabbit AlexaFluor 488
Secondary antibody dilution: 1:1000

ATP6V0A2 antibody - N-terminal region (ARP42365_P050) validated by WB using HeLa cells at 1:300.

Application: Western blotting
Species+tissue/cell type: HeLa cells
How many ugsof tissue/cell lysate run on the gel:1. 10 ug untransfected HeLa lysate2. 10 ug mATP6V0A2 (Partial) transfected HeLa lysate3. 10 ug mATP6V0A2-FLAG transfected HeLa lysate4. 10 ug mATP6V0A1-FLAG transfected HeLa lysate
Primary antibody dilution: 1:300
Secondary antibody: Anti-rabbit-HRP
Secondary antibody dilution: 1:1000

Host: Rabbit
Target: ATP6V0A2
Positive control (+): MCF7 (N10)
Negative control (-): HEK293 (HEK293)
Antibody concentration: 1ug/ml

ATP6V0A2 antibody - N-terminal region (ARP42365_P050) in Human HeLa using Western Blot