UBE3A Antibody - middle region

Product Overview

• Article Name: UBE3A Antibody - middle region
• Biozol Catalog Number: ASB-ARP42951_P050
• Supplier Catalog Number: ARP42951_P050
• Alternative Catalog Number: ASB-ARP42951_P050-25UL,ASB-ARP42951_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE3A
• Alternative Names: AS, ANCR, E6-AP, HPVE6A, EPVE6AP

Product Description

UBE3A is an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 101kDa
• NCBI: 7337
• UniProt: Q05086
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-UBE3A Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Hela cell lysateUBE3A is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells

Lanes:
Lane 1: 7ug HeLa lysate+EGFP SiRNA
Lane 2: 7ug HeLa lysate+UBE3A SiRNA
Primary Antibody Dilution:
1:300
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:500
Gene Name:
UBE3A
Submitted by:
Seiji Masuda, Kitashirakawa Oiwakecho, Kyoto University

Host: Rabbit
Target Name: UBE3A
Sample Tissue: Mouse Kidney
Antibody Dilution: 1ug/ml

Host: Mouse
Target Name: UBE3A
Sample Tissue: Mouse Kidney
Antibody Dilution: 1ug/ml

UBE3A antibody - middle region (ARP42951_P050) in Human HeLa using Western Blot