UBE2D3 Antibody - N-terminal region

Product Overview

• Article Name: UBE2D3 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP43009_P050
• Supplier Catalog Number: ARP43009_P050
• Alternative Catalog Number: ASB-ARP43009_P050-25UL,ASB-ARP43009_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBE2D3
• Alternative Names: UBC4/5, UBCH5C, E2(17)KB3

Product Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Multiple spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 17kDa
• NCBI: 7323
• UniProt: P61077
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-UBE2D3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: MCF7 cell lysate.UBE2D3 is strongly supported by BioGPS gene expression data to be expressed in MCF7

Lanes:
1: 40ng HIS-UBE2D1 protein
2: 40ng HIS-UBE2D2 protein
3: 40ng HIS-UBE2D3 protein
4: 40ng HIS-UBE2D4 protein
5: 40ng HIS-UBE2E1 protein
6: 40ng HIS-UBE2E2 protein
7: 40ng HIS-UBE2E3 protein
8: 40ng HIS-UBE2K protein
9: 40ng HIS-UBE2L3 protein
10: 40ng HIS-UBE2N protein
11: 40ng HIS-UBE2V1 protein
12: 40ng HIS-UBE2V2 protein.
Primary Antibody Dilution:
1:500
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:50,000
Gene Name:
UBE2D3
Submitted by:
Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK.

Host: Rabbit
Target Name: UBE2D3
Sample Type: Jurkat
Antibody Dilution: 1.0ug/mlUBE2D3 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

Host: Rabbit
Target Name: UBE2D3
Sample Type: MCF7
Antibody Dilution: 1.0ug/mlUBE2D3 is strongly supported by BioGPS gene expression data to be expressed in MCF7

Host: Rabbit
Target Name: UBE2D3
Sample Type: Human Fetal Lung
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: UBE2D3
Sample Type: Human Fetal Liver
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: UBE2D3
Sample Type: 293T
Antibody Dilution: 1.0ug/mlUBE2D3 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells

UBE2D3 antibody - N-terminal region (ARP43009_P050) in Human MCF-7 using Western Blot