UBE2M Antibody - N-terminal region

Catalog Number: ASB-ARP43030_P050
Article Name: UBE2M Antibody - N-terminal region
Biozol Catalog Number: ASB-ARP43030_P050
Supplier Catalog Number: ARP43030_P050
Alternative Catalog Number: ASB-ARP43030_P050-25UL,ASB-ARP43030_P050-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: IHC, WB
Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBE2M
Alternative Names: UBC12, hUbc12, UBC-RS2
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. It is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 21kDa
NCBI: 9040
UniProt: P61081
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sample Type:ANT2 cells
Red:AAntibody
Blue:ADAPI
Primary Dilution:A1ug/50ul antibody
Secondary Antibody:AAlexa goat anti-rabbit 594
Image Submitted by:AYuzhi Chen, University of Arkansas for Medical Sciences A
Sample Type:
Human brain stem cellsPrimary Antibody Dilution:
1:500Secondary Antibody:
Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution:
1:1000Color/Signal Descriptions:
UBE2M: Red DAPI:BlueGene Name:
UBE2M Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science
WB Suggested Anti-UBE2M Antibody Titration: 0.2-1 ug/ml
Positive Control: 721_B cell lysateUBE2M is supported by BioGPS gene expression data to be expressed in 721_B
Sample Type:2. mouse brain extracts (80ug)
Primary Antibody Dilution:A2ug/ml
Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution:A1: 20,000
Image Submitted by: Yuzhi Chen
University of Arkansas for Medical ScienceA
Host: Rabbit
Target Name: UBE2M
Sample Type: Human MCF7
Antibody Dilution: 1.0ug/mlUBE2M is supported by BioGPS gene expression data to be expressed in MCF7
Host: Rabbit
Target Name: UBE2M
Sample Type: Human 721_B
Antibody Dilution: 1.0ug/mlUBE2M is supported by BioGPS gene expression data to be expressed in 721_B
UBE2M antibody - N-terminal region (ARP43030_P050) in Human 721_B using Western Blot