STUB1 Antibody - C-terminal region

Product Overview

• Article Name: STUB1 Antibody - C-terminal region
• Biozol Catalog Number: ASB-ARP43059_P050
• Supplier Catalog Number: ARP43059_P050
• Alternative Catalog Number: ASB-ARP43059_P050-25UL,ASB-ARP43059_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human STUB1
• Alternative Names: CHIP, SCA48, UBOX1, SCAR16, HSPABP2, NY-CO-7, SDCCAG7

Product Description

STUB1 modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It has E3 ubiquitin-protein ligase activity and targets misfolded chaperone substrates towards proteasomal degradation. STUB1 mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 35kDa
• NCBI: 10273
• UniProt: Q9UNE7
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-STUB1 Antibody
Catalog Number: ARP43059_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: N/A
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

WB Suggested Anti-STUB1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Transfected 293T

Lanes:
1:1ug insoluble STUB1 protein, 2:1ug soluble STUB1 protein, 3:1ug EPM2A protein, 4:1ug insoluble PPP1R3C protein, 5:1ug soluble PPP1R3C protein
Primary Antibody Dilution:
1:2500
Secondary Antibody:
Anti-rabbit-AP
Secondary Antibody Dilution:
1:20,000
Gene Name:
STUB1
Submitted by:
Pedro Castanheira, Biocant

Lanes:
Lane 1: 40ug canine retina lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Goat anti-rabbit-HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
STUB1
Submitted by:
Dr. Stefania Marsili and Dr. William Beltran, University of Pennsylvania

STUB1 antibody - C-terminal region (ARP43059_P050) in Transfected 293T using Western Blot