HERC5 Antibody - middle region

Product Overview

• Article Name: HERC5 Antibody - middle region
• Biozol Catalog Number: ASB-ARP43213_P050
• Supplier Catalog Number: ARP43213_P050
• Alternative Catalog Number: ASB-ARP43213_P050-25UL,ASB-ARP43213_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Equine, Human, Porcine, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HERC5
• Alternative Names: CEB1, CEBP1

Product Description

HERC5 is a member of the HERC family of ubiquitin ligases.It contains a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulates expression of HERC5 protein in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets.This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 117kDa
• NCBI: 51191
• UniProt: Q69G20
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. An isoform containing the peptide sequence is present at 64 kDa.

Rabbit Anti-HERC5 Antibody
Catalog Number: ARP43213_P050
Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue
Observed Staining: Cytoplasmic in cell bodies and processes of pinealocytes
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1/600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

WB Suggested Anti-HERC5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate

HERC5 antibody - middle region (ARP43213_P050) validated by WB using hek293 cell lysate at 1:1000.HERC5 is supported by BioGPS gene expression data to be expressed in HEK293

HERC5 antibody - middle region (ARP43213_P050) in Human HepG2 using Western Blot