SLC6A5 Antibody - N-terminal region

Product Overview

• Article Name: SLC6A5 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP43872_P050
• Supplier Catalog Number: ARP43872_P050
• Alternative Catalog Number: ASB-ARP43872_P050-25UL,ASB-ARP43872_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of human SLC6A5
• Alternative Names: NET1, GLYT2, HKPX3, GLYT-2

Product Description

This gene encodes a sodium- and chloride-dependent glycine neurotransmitter transporter. This integral membrane glycoprotein is responsible for the clearance of extracellular glycine during glycine-mediated neurotransmission. This protein is found in glycinergic axons and maintains a high presynaptic pool of neurotransmitter at glycinergic synapses. Mutations in this gene cause hyperekplexia, a heterogenous neurological disorder characterized by exaggerated startle responses and neonatal apnea. Two transcript variants encoding different isoforms have been found for this gene.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 43 kDa
• NCBI: 9152
• UniProt: Q9Y345
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-SLC6A5 Antibody
Catalog Number: ARP43872_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy2/3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 a€'' 2.0 sec
Protocol located in Reviews and Data.

Host: Rabbit
Target Name: SLC6A5
Sample Type: COLO205 Whole cell lysates
Antibody Dilution: 1.0ug/ml

WB Suggested Anti-SLC6A5 antibody Titration: 1 ug/mL
Sample Type: Human heart

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 0.5, 5, and 50 ug/mL. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for either two or three concentrations were averaged and results and standard deviation are shown.

SLC6A5 Antibody - N-terminal region (ARP43872_P050) in Human COLO205 using Western Blot