SLC25A28 Antibody - C-terminal region

Product Overview

• Article Name: SLC25A28 Antibody - C-terminal region
• Biozol Catalog Number: ASB-ARP44054_P050
• Supplier Catalog Number: ARP44054_P050
• Alternative Catalog Number: ASB-ARP44054_P050-25UL,ASB-ARP44054_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SLC25A28
• Alternative Names: MFRN2, MRS4L, MRS3/4, NPD016

Product Description

SLC25A28 is a mitochondrial iron transporter that mediates iron uptake. It is probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells. The iron delivered into the mitochondria, presumably as Fe(2+), is then probably delivered to ferrochelatase to catalyze Fe(2+) incorporation into protoprophyrin IX to make heme.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 39 kDa
• NCBI: 81894
• UniProt: Q96A46
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Immunohistochemistry with Human kidney lysate tissue at an antibody concentration of 5.0ug/ml using anti-SLC25A28 antibody (ARP44054_P050)

WB Suggested Anti-SLC25A28 Antibody Titration: 1 ug/ml
Positive Control: MCF-7 whole cell lysates

Host: Rabbit
Target: SLC25A28
Positive control (+): Human stomach (ST)
Negative control (-): HepG2 (HG)
Antibody concentration: 1ug/ml

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

SLC25A28 antibody - C-terminal region (ARP44054_P050) in Human MCF-7 using Western Blot