SLC5A8 Antibody - middle region

Catalog Number: ASB-ARP44110_P050
Article Name: SLC5A8 Antibody - middle region
Biozol Catalog Number: ASB-ARP44110_P050
Supplier Catalog Number: ARP44110_P050
Alternative Catalog Number: ASB-ARP44110_P050-25UL,ASB-ARP44110_P050-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: WB
Species Reactivity: Human, Mouse, Rabbit, Rat
Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SLC5A8
Alternative Names: AIT, SMCT, SMCT1
SLC5A8 has been shown to transport iodide by a passive mechanism (Rodriguez et al., 2002 [PubMed 12107270]) and monocarboxylates and short-chain fatty acids by a sodium-coupled mechanism (Gopal et al., 2004 [PubMed 15322102]). In kidney, SLC5A8 functions
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 66 kDa
NCBI: 160728
UniProt: Q8N695
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 0.5 ug/mL of the antibody was used in this experiment. Protein is glycosylated as well.
WB Suggested Anti-SLC5A8 Antibody Titration: 0.2-1 ug/ml
Positive Control: OVCAR-3 cell lysate
Host: Rabbit
Target Name: SLC5A8
Sample Tissue: Human OVCAR-3
Antibody Dilution: 1.0ug/ml
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
Western Blot