PLD3 Antibody - N-terminal region

Catalog Number: ASB-ARP44768_P050
Article Name: PLD3 Antibody - N-terminal region
Biozol Catalog Number: ASB-ARP44768_P050
Supplier Catalog Number: ARP44768_P050
Alternative Catalog Number: ASB-ARP44768_P050-25UL,ASB-ARP44768_P050-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: WB
Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PLD3
Alternative Names: AD19, HUK4, HU-K4, SCA46
PLD3 is a ingle-pass type II membrane protein. It belongs to the phospholipase D family. PLD3 contains 2 PLD phosphodiesterase domains. The exact function of PLD3 remains unknown.
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 55 kDa
NCBI: 23646
UniProt: Q8IV08
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
WB Suggested Anti-PLD3 Antibody Titration: 0.2-1 ug/ml
Positive Control: Human heart
Host: Rabbit
Target Name: PLD3
Sample Type: Human Fetal Heart
Antibody Dilution: 1.0ug/ml
Host: Rabbit
Target Name: PLD3
Sample Tissue: Human Fetal Lung
Antibody Dilution: 1.0ug/ml
Host: Rabbit
Target: PLD3
Positive control (+): Human stomach (ST)
Negative control (-): HepG2 (HG)
Antibody concentration: 3ug/ml
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
PLD3 antibody - N-terminal region (ARP44768_P050) in Human Fetal Lung using Western Blot