ATP6V0C Antibody - middle region

Product Overview

• Article Name: ATP6V0C Antibody - middle region
• Biozol Catalog Number: ASB-ARP45161_P050
• Supplier Catalog Number: ARP45161_P050
• Alternative Catalog Number: ASB-ARP45161_P050-25UL,ASB-ARP45161_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Human, Porcine, Rabbit, Rat, Sheep
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATP6V0C
• Alternative Names: ATPL, VATL, VPPC, Vma3, ATP6C, ATP6L

Product Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c, c, and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is part of the V0 domain. This gene had the previous symbols of ATP6C and ATP6L.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 16 kDa
• NCBI: 527
• UniProt: P27449
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-ATP6V0C Antibody Titration: 0.2-1 ug/ml
Positive Control: OVCAR-3 cell lysate.ATP6V0C is strongly supported by BioGPS gene expression data to be expressed in OVCAR3

Host: Rabbit
Target Name: ATP6V0C
Sample Type: ACHN
Antibody Dilution: 1.0ug/mlATP6V0C is strongly supported by BioGPS gene expression data to be expressed in Human ACHN cells

Host: Rabbit
Target Name: ATP6V0C
Sample Type: Human Lung
Antibody Dilution: 1.0 ug/ml

Host: Rabbit
Target Name: ATP6V0C
Sample Tissue: Human RPMI 8226 Whole Cell
Antibody Dilution: 1ug/ml

Host: Rabbit
Target: ATP6V0C
Positive control (+): RPMI-8226 (N12)
Negative control (-): U937 (N31)
Antibody concentration: 1ug/ml

Host: Rabbit
Target Name: ATP6V0C
Sample Tissue: Human COLO205 Whole Cell
Antibody Dilution: 3ug/ml

25 ug of the indicated whole cell extracts was loaded onto a 10-20% gradient SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Recommended antibody dilution is 1 ug/mL to 3 ug/mL.

ATP6V0C antibody - middle region (ARP45161_P050) in Human Lung tissue using Western Blot