SYP Antibody - N-terminal region

Product Overview

• Article Name: SYP Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP45435_P050
• Supplier Catalog Number: ARP45435_P050
• Alternative Catalog Number: ASB-ARP45435_P050-25UL,ASB-ARP45435_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Goat, Guinea pig, Human, Mouse, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SYP
• Alternative Names: MRX96, MRXSYP

Product Description

Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells. Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 34kDa
• NCBI: 6855
• UniProt: B2R7L6
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

rat and mouse brainDilution: 10ug/mlObserved:staining in synaptic puncta in many parts of brain

Application: Immunohistochemistry
Species+tissue/cell type: Mouse Gut Tissue TgWnt1-Cre/+ Ednrbflex3/+ Rosa26YFPStop/YFPStop
How many ugsof tissue/cell lysate run on the gel: 11 m Mouse Gut Tissue
Primary antibody dilution: 1:50
Secondary antibody: Goat anti-rabbit-cy3
Secondary antibody dilution: 1:1500

WB Suggested Anti-SYP Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Transfected 293T

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

SYP antibody - N-terminal region (ARP45435_P050) in Human 293T using Western Blot