PPAT Antibody - N-terminal region

Product Overview

• Article Name: PPAT Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP46079_P050
• Supplier Catalog Number: ARP46079_P050
• Alternative Catalog Number: ASB-ARP46079_P050-25UL,ASB-ARP46079_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PPAT
• Alternative Names: GPAT, PRAT, ATASE

Product Description

PPAT is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. Its gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4.The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 57 kDa
• NCBI: 5471
• UniProt: Q06203
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Human kidney

PPAT antibody - N-terminal region (ARP46079_P050) validated by WB using HepG2 Cell Lysate at 0.25ug/ml.PPAT is supported by BioGPS gene expression data to be expressed in HepG2

Sample Type:A1. Human Skin Fibroblasts (100ug)
2. HepG2 cells (20ug)
3. HEK273 cells (20ug)
4. HeLa skin cells(20ug)
5. Hamster CHO K1 cells (20ug)
Primary Dilution:A1:5000
Secondary Antibody:AClean-Blot IP detection Reagent and Kit
Secondary Dilution:A1:2000
Image Submitted By:AV. Skopova, M. Zikanova
Institute of Inherited Metabolic Disorders, Charles University in Prague and General Hopspital in PragueA

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.

Host: Rabbit
Target Name: PPAT
Sample Tissue: Human 293T Whole Cell
Antibody Dilution: 1ug/ml

PPAT antibody - N-terminal region (ARP46079_P050) in Human HepG2 using Western Blot