PSME3 Antibody - N-terminal region

Product Overview

• Article Name: PSME3 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP46142_P050
• Supplier Catalog Number: ARP46142_P050
• Alternative Catalog Number: ASB-ARP46142_P050-25UL,ASB-ARP46142_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human PSME3
• Alternative Names: Ki, PA28G, HEL-S-283, PA28gamma, REG-GAMMA, PA28-gamma

Product Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME3 is the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 30 kDa
• NCBI: 10197
• UniProt: P61289-2
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Anti-PSME3 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Rabbit Anti-PSME3 Antibody
Catalog Number: ARP46142
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 0.5 ug/mL of the antibody was used in this experiment. The protein may be modified by phosphorylation.

WB Suggested Anti-PSME3 Antibody Titration: 1 ug/ml
Positive Control: HepG2 cell lysatePSME3 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells

PSME3 Antibody - N-terminal region (ARP46142_P050) in Human HepG2 using Western Blot